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Sterilization (microbiology)

life science Maturity 9-11 Next article →

Sterilization is a way to remove or kill all life on a surface or in a liquid.

Contamination on agar plate
Contamination on agar plate

This includes tiny living things like bacteria, fungi, and viruses. It also stops biological agents called prions. Sterilization is different from other cleaning methods like disinfection. Disinfection only reduces the number of germs. Sterilization must eliminate all forms of life to be successful. When an object is completely clean this way, we call it sterile or aseptic.

Curette in sterile packaging
Curette in sterile packaging

There are many ways to make things sterile. One common way is using heat, such as steam or dry heat. Some people use chemicals or special light called irradiation. High pressure can also work to kill germs. Scientists even use filtration to remove tiny organisms. For tools that cannot handle heat, we use gases. One example is ethylene oxide gas, which has been used since the 1950s.

Ethylene oxide sterilisation sticker on box of medical supplies
Ethylene oxide sterilisation sticker on box of medical supplies

Humans have used heat to keep things safe for a long time. Nicolas Appert was one of the first to help modernize this. He discovered that applying heat for a certain amount of time slowed down food decay. This discovery led to the canning of foods we use today. Canning helps reduce food poisoning by keeping liquids and foods safe to eat. Other food methods include using very high temperatures or high pressure, which is called pascalization.

Joseph Lister c1855
Joseph Lister c1855

Sterilization is very important in hospitals and medicine. Tools like scalpels and needles must be sterile before they touch a person's skin or blood. Many medical tools are made of materials that can go into a steam machine called an autoclave. This machine uses pressurized steam to kill germs quickly. However, many new tools are made of plastic. These plastics need low-temperature sterilization so they do not melt.

2003-12-03-Chemiclav
2003-12-03-Chemiclav

We even use sterilization to protect our solar system. There are strict rules to stop Earth germs from touching other planets. This is important for missions to places that might support life. Since some spacecraft tools cannot handle high heat, scientists use other ways. They might use ultraviolet light or chemical sterilization. This keeps our space tools clean and protects other worlds from our tiny germs.

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🖼️ Images & Media (7)
File:Contamination on agar plate.jpg
Contamination on agar plate.jpg
File:2003-12-03-Heissluft-Sterilisator.JPG
2003-12-03-Heissluft-Sterilisator.JPG
File:Ethylene oxide sterilisation sticker on box of medical supplies.jpg
Ethylene oxide sterilisation sticker on...
File:E-beam-x-ray-gamma-efficiency.jpg
E-beam-x-ray-gamma-efficiency.jpg
File:2003-12-03-Chemiclav.JPG
2003-12-03-Chemiclav.JPG
File:Curette in sterile packaging.jpg
Curette in sterile packaging.jpg
File:Joseph Lister c1855.jpg
Joseph Lister c1855.jpg
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