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Infrared spectroscopy

physical science Maturity 11-13 Next article →

Infrared spectroscopy, often called IR spectroscopy, is a powerful analytical technique. It measures how infrared radiation interacts with matter through absorption, emission, or reflection. Scientists use this method to identify chemical substances or functional groups. It works for samples in solid, liquid, or gaseous forms. This tool helps characterize new materials and verify known substances.

Osiris-Rex Ovirs gsfc 20150619 2015-12655 019-023
Osiris-Rex Ovirs gsfc 20150619 2015-12655 019-023

The mechanism relies on the unique way molecules vibrate. Every molecule has a specific structure and mass. These atoms are held together by bonds that act like springs. When infrared light hits a molecule, absorption occurs at resonant frequencies. This happens when the light's frequency matches the molecule's vibrational frequency.

Bromomethane
Bromomethane

The energy of these vibrations depends on the atomic masses and the shape of the molecular potential energy surfaces. For a vibration to be "IR active," it must cause a change in the molecule's electric dipole moment. This means the distribution of electric charge must shift as the atoms move.

An infrared spectrum is the data produced by an infrared spectrometer. This instrument, such as a Fourier transform infrared (FTIR) spectrometer, creates a visual graph. The vertical axis of the graph shows light absorbance or transmittance. The horizontal axis represents frequency, wavelength, or wavenumber. Scientists often use reciprocal centimeters, symbolized as cm⁻¹, for the wavenumber.

Bromomethane IR spectroscopy
Bromomethane IR spectroscopy
IR-spectroscopy-sample
IR-spectroscopy-sample

The spectrum acts as a chemical fingerprint for the sample.

The electromagnetic spectrum is divided into three main infrared regions. The near-infrared region has high energy, ranging from 14,000 to 4,000 cm⁻¹. It can excite overtone or combination modes of molecular vibrations. The mid-infrared region spans from 4,000 to 400 cm⁻¹. This is the most common region for studying fundamental vibrations and rotational-vibrational structures.

Symmetrical stretching
Symmetrical stretching

The far-infrared region has lower energy, between 400 and 10 cm⁻¹. It is used for rotational spectroscopy and low-frequency vibrations. There is also a terahertz region from 2 to 130 cm⁻¹ that probes intermolecular vibrations.

Molecules possess different types of vibrational modes. For a molecule with N atoms, linear molecules have 3N–5 modes. Nonlinear molecules have 3N–6 modes, also called degrees of freedom. These modes include stretching and bending. Stretching can be symmetric or antisymmetric. Bending modes include scissoring, rocking, wagging, and twisting.

Co2 vibrations
Co2 vibrations
Scissoring
Scissoring
Wagging
Wagging

Complex molecules with many bonds produce very complex spectra with many peaks.

This technology has many vital applications across various industries. In organic and inorganic chemistry, it is a reliable tool for research. The food industry uses it to measure compound concentrations. In environmental monitoring, infrared gas analyzers track CO2 levels in greenhouses. It is also used in gas leak detection for natural gas and crude oil transportation.

Mary Moffit using Infrared spectrophotometer 2012 017 b1f6 79407z11s
Mary Moffit using Infrared spectrophotometer 2012 017 b1f6 79407z11s

In recycling, it helps sort different plastic polymers like PET and HDPE.

Advanced research is pushing the limits of this science. Scientists are coupling IR spectroscopy with machine learning and artificial intelligence. This combination shows promise for the rapid sensing of bacteria. It could help differentiate bacteria at the genus, species, or serotype levels. This could lead to faster antimicrobial susceptibility testing in clinical settings. Future developments may even include miniature spectrometers linked to cloud databases or chips inside smartphones.

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🖼️ Images & Media (17)
File:Osiris-Rex Ovirs gsfc 20150619 2015-12655 019-023.jpg
Osiris-Rex Ovirs gsfc 20150619 2015-12655...
Mary Moffit using Infrared...
File:Portable Screening Devices (1435) (8225044148).jpg
Portable Screening Devices (1435) (8225044148).jpg
File:Bromomethane IR spectroscopy.svg
Bromomethane IR spectroscopy.svg
File:Bromomethane.gif
Bromomethane.gif
File:Co2 vibrations.svg
Co2 vibrations.svg
File:Symmetrical stretching.gif
Symmetrical stretching.gif
File:Asymmetrical stretching.gif
Asymmetrical stretching.gif
File:Scissoring.gif
Scissoring.gif
File:Modo rotacao.gif
Modo rotacao.gif
File:Wagging.gif
Wagging.gif
File:Twisting.gif
Twisting.gif

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