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Dictyostelium discoideum

life science Maturity 9-11 Next article →

Dictyostelium discoideum is a species of soil-dwelling amoeba. It belongs to the phylum Amoebozoa and the infraphylum Mycetozoa. This organism is part of a group called the slime molds. This name describes how it switches between single-celled and multicellular forms. It is a eukaryote with a unique asexual life cycle. This cycle consists of four distinct stages: vegetative, aggregation, migration, and culmination. Because of its simple life cycle, scientists use it as a model organism. It helps researchers study genetic, cellular, and biochemical processes in many other organisms.

Dicty Life Cycle H01
Dicty Life Cycle H01

The life cycle begins when spores are released from a mature sorocarp. A sorocarp is a type of fruiting body. Under warm and moist conditions, myxamoebae hatch from these spores. During the vegetative stage, these myxamoebae divide by mitosis to grow. They feed on bacteria like Escherichia coli found in decaying organic matter. The bacteria actually secrete folic acid, which attracts the myxamoebae to their food. This stage continues as long as the bacteria are plentiful.

Life-cycle of farmer dictyostelium discoideum
Life-cycle of farmer dictyostelium discoideum

When the bacteria supply runs out, the amoebae enter the aggregation stage. Starvation triggers the production of specific protein compounds. One important protein is adenylyl cyclase, which creates a chemical called cyclic AMP. The amoebae secrete this cyclic AMP to attract neighboring cells. This process is known as chemotaxis, which is movement toward a chemical signal. As cells move toward the signal, they bump into each other. They use glycoproteins, which are adhesion molecules, to stick together.

Dictyostelium Late Aggregation 2
Dictyostelium Late Aggregation 2

Once the cells form a tight aggregate, the migration stage begins. The mound of cells tips over and lies flat on the ground. The amoebae now work together as a single motile pseudoplasmodium, also called a slug. This slug is about 2 to 4 mm long. It can be composed of up to 100,000 individual cells. The slug moves in a forward-only direction toward light, heat, or humidity. It produces a cellulose sheath in its anterior cells to help it move. As it travels, the slug leaves a slimy trail behind it.

Inside the slug, cells begin to undergo differentiation. Differentiation is the process where cells become specialized for specific roles. Cyclic AMP and a differentiation-inducing factor help form different cell types. The slug divides into prestalk cells and prespore cells. The prestalk cells move to the anterior, or front, end. The prespore cells move to the posterior, or back, end. Recently, scientists also discovered anterior-like cells in the posterior region. These cells help form the very bottom of the fruiting body and the spore caps.

The culmination stage starts once the slug finds a suitable environment. The slug settles and the ends spread out to look like a "Mexican hat." During this stage, the prestalk and prespore cells switch positions. The anterior end forms a cellulose tube to create a stalk. This tube allows the posterior cells to move up the outside. The prestalk cells move down to form the base. This rearrangement takes about 8 to 10 hours to complete. The final fruiting body is 1 to 2 mm tall.

Dicty Life Cycle H01
Dicty Life Cycle H01

While asexual reproduction is common, Dictyostelium can also reproduce sexually. This occurs when specific mating types meet in dark, wet environments. There are three different mating types, identified by specific genes like MatA, MatS, and MatT. Successful mating results in the formation of a diploid zygote. In some cases, this forms a macrocyst, which is a thick, protective structure. Inside the macrocyst, the cell undergoes meiosis and mitosis to produce new amoebae. This recombination is very important for natural populations of the species.

Scientists value Dictyostelium because it is a powerful model organism. Many of its genes are homologous, or similar, to human genes. It is used to study cell differentiation, chemotaxis, and apoptosis. It also helps researchers understand cell sorting and signal transduction. Studying its chemotaxis can provide insights into human conditions like asthma and arthritis. Because it is closely related to higher metazoans, it is an excellent tool for biology. It allows us to observe the evolution from single cells to multicellular life.

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File:Dicty Life Cycle H01.svg
Dicty Life Cycle H01.svg
File:Dictyostelium Late Aggregation 2.JPG
Dictyostelium Late Aggregation 2.JPG
File:Life-cycle of farmer dictyostelium discoideum.jpg
Life-cycle of farmer dictyostelium discoideum.jpg
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