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Biological carbon fixation

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Biological carbon fixation is the fundamental process of converting inorganic carbon into organic compounds. This process, also known as carbon assimilation, allows living things to create the building blocks for life. These organic compounds serve as energy storage and as the structural components for other biomolecules. Carbon is an essential base element for all organic life. Through fixation, carbon moves from the atmosphere, oceans, and lithosphere into the biosphere. This movement is a key part of the global carbon cycle. Understanding these cycles is vital for studying ecosystem dynamics and climate regulation.

Organisms that can fix their own carbon are called autotrophs. These are divided into two main groups based on their energy source. Photoautotrophs, such as most plants and algae, use sunlight to drive the process through photosynthesis. Lithoautotrophs use inorganic oxidation to obtain energy. In contrast, heterotrophs, including animals and fungi, cannot fix carbon themselves. They must grow by consuming the organic carbon produced by autotrophs or other heterotrophs.

Carbon fixation cycles
Carbon fixation cycles

The Calvin cycle is the most dominant mechanism for carbon fixation on Earth. It accounts for approximately 90% of all biological carbon fixation. In plants, this cycle occurs within the chloroplasts. It is also the primary method used by algae, cyanobacteria, and certain purple bacteria. The cycle works by consuming adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide phosphate (NADPH). These molecules provide the energy and electrons needed to convert carbon dioxide into a sugar called triose phosphate (TP). This sugar is composed of glyceraldehyde 3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP).

Calvin Cycle 5
Calvin Cycle 5

Other pathways allow life to thrive in environments without sunlight. The reverse Krebs cycle, or reductive citric acid cycle, is one such method. It was discovered in 1966 by scientists Evans, Buchanan, and Arnon while studying the green sulfur bacterium Chlorobium limicola. This cycle is used by certain anaerobic bacteria and archaea. It is especially important in the aphotic, or dark, zones of the ocean. In hydrothermal vents, Campylobacterota use this cycle to enable primary production. Without this dark primary production, these deep-sea habitats could not support life.

Reductive TCA cycle
Reductive TCA cycle

The reductive acetyl-CoA pathway, also known as the Wood-Ljungdahl pathway, is another specialized method. This pathway uses carbon dioxide as an electron acceptor and hydrogen as an electron donor to form acetic acid. It is widespread among the phylum Bacillota and is used by methanogens in the archaea domain. This pathway is remarkably efficient for organisms living in low-energy, anaerobic conditions. It requires only one molecule of ATP to produce one molecule of pyruvate. This efficiency makes it a primary choice for chemolithoautotrophs in harsh environments.

Reduktiver Acetyl-CoA-Weg
Reduktiver Acetyl-CoA-Weg

Scientists have identified several other complex pathways used by microorganisms. The 3-hydroxypropionate (3-HP) bicycle was discovered in 1989 in the Chloroflexaceae family. This pathway involves 19 different reactions and uses 13 multifunctional enzymes to fix three bicarbonate molecules. It is a costly process, consuming 7 ATP for pyruvate synthesis and 3 ATP for phosphate triose. Variants of this cycle exist, such as the 3-HP/4-hydroxybutyrate cycle found in the archaeon Metallosphaera sedula. Other versions include the dicarboxylate/4-hydroxybutyrate cycle, which was proposed for the hyperthermophile Ignicoccus hospitalis in 2008.

The scale of carbon fixation is massive and affects the entire planet. It is estimated that photosynthesis converts about 250 billion tons of carbon dioxide annually. Nearly half of this occurs in the oceans, while the rest happens in terrestrial environments, mostly in the tropics. The gross amount of fixed carbon is even higher because about 40% is consumed by respiration immediately after fixation. Since the origin of life, it is estimated that 200 billion billion tons of carbon have been fixed.

CO2FixnData
CO2FixnData

Carbon fixation is deeply connected to the chemistry of the Earth. Some enzymes, like RuBisCO, exhibit carbon isotope discrimination. This means they prefer the lighter carbon-12 isotope over the heavier carbon-13 isotope. Measuring these isotopic ratios helps scientists evaluate plant water use efficiency. It also helps them track the sources of carbon within the global cycle. From the microscopic bacteria in the soil to the massive forests of the tropics, these chemical pathways sustain the biological world.

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Metabolic Metro Map (no legends).svg
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Calvin Cycle 5.svg
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