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Bacteroidota

life science Maturity 11-13 Next article →

Bacteroidota is a massive phylum of bacteria that plays a vital role in Earth's ecosystems. These organisms are Gram-negative, meaning they have a specific cell wall structure. They are also nonsporeforming, so they do not create tough protective shells to survive harsh conditions. Most species are rod-shaped and can live in environments without oxygen, known as anaerobic, or with oxygen, known as aerobic. They are found in many places, including soil, sediments, and seawater.

Many of these bacteria live in the guts of animals, where they are highly abundant. In the intestines, they can reach concentrations of 10^11 cells per gram of material.

Inside the gastrointestinal tract, Bacteroidota perform essential metabolic conversions for their hosts. They are highly adjusted to this environment and are considered a stable part of the gut microflora. These bacteria are often saccharolytic, which means they break down complex sugar polymers like starch, cellulose, xylans, and pectins. Other species are asaccharolytic, meaning they do not rely on sugar for energy. Some also use proteases, which are enzymes, to perform proteolytic activity to degrade proteins.

Through these processes, they produce major end-products like succinic acid, acetic acid, and sometimes propionic acid. This metabolic work helps provide nutrients that the host animal needs to survive.

Bacteroidota are categorized into different classes based on where they live and how they behave. The class Bacteroidia is the most well-studied group. It includes the genus Bacteroides, which is common in the feces of warm-blooded animals, and Porphyromonas, which lives in the human oral cavity.

Other classes, such as Flavobacteriales and Sphingobacteriales, consist of typical soil bacteria. These soil-dwelling types are only occasionally found in the human gut. However, some specific types like Capnocytophaga and Sphingobacterium can be found in the human mouth. This shows how the phylum is divided into many specialized groups.

Scientific understanding of this group has evolved significantly since the late 19th century. In 1898, the species Bacteroides fragilis was the first member of this group isolated as a human pathogen. It was linked to clinical cases of appendicitis. For a long time, scientists believed most Gram-negative gut bacteria belonged to the genus Bacteroides. However, recent research has led to many species undergoing reclassification. Today, most gastrointestinal Bacteroidota belong to families like Bacteroidaceae, Prevotellaceae, Rikenellaceae, and Porphyromonadaceae.

These bacteria also have unique physical traits and specialized habitats. Many members of the genera Flexibacter and Cytophaga appear yellow-orange or pink-red. This color comes from pigments called flexirubins, or sometimes carotenoids in marine species. In extreme environments, the genus Salinibacter lives in hypersaline lakes. These bacteria live in salt-saturated brines and are so similar to halophilic Archaea that they were difficult to identify for a long time.

This shows the incredible diversity of how Bacteroidota can adapt to different chemical conditions.

Modern science uses genomics to study the deep connections between these organisms. Researchers have identified 27 proteins that serve as molecular markers for the phylum. One protein is found in every sequenced Bacteroidota species. Other proteins are missing in certain groups, like the genus Bacteroides, likely due to selective gene loss. Scientists also look for a conserved signature indel, which is a specific three-amino-acid deletion in the ClpB chaperone protein.

These genetic signatures help scientists map the history of the bacteria.

Bacteroidota are part of a much larger evolutionary group known as the FCB superphylum. This group includes the phyla Chlorobiota and Fibrobacterota. Phylogenetic trees, which show evolutionary relationships, show that these three phyla branch very closely together. They share unique proteins and specific signature indels in proteins like RpoC. These shared traits provide compelling evidence that they all descended from a single common ancestor. This connection links many different types of bacteria across the tree of life.

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