Male frogs have a skin pouch. 

Most male frogs have a skin pouch. 

Most male frogs and toads have a vocal sac. 

Most male frogs and toads have a vocal sac. 

To make a sound, the frog follows a few steps. First, the frog fills its lungs with air. Next, it shuts its nose and its mouth. Then, it pushes the air out of its lungs. The air goes through the larynx. This is a part that creates vibrations. The air moves into the vocal sac. The sound vibrates on the elastic skin. This is called amplification. It makes the sound much louder. 
Many species grow their sacs in a similar way. A single-lobed sac starts as two small growths. These growths are on the floor of the mouth. They grow into two small pouches. These pouches expand until they meet in the middle. They form one large cavity. This cavity grows until it is fully developed. This is how the pouch becomes ready to use.
Some frogs do not have these sacs. These frogs often live near flowing water. The sound of the water is very loud. It can overpower the frog's call. Without a sac, a frog is heard only a few metres away. Some frogs with sacs can be heard much further. 
Some frogs use the sac for a different job. This happens in the Rhinodermatidae family. Males scoop up newly hatched tadpoles into their mouths. The tadpoles then move into the vocal sac. In Darwin's frog, they stay there until metamorphosis. Metamorphosis is when they change into adults. The Chile Darwin's frog moves them to water. 
A vocal sac is a flexible membrane made of skin. Most male frogs and toads possess this special feature. It serves as a vital tool for animal communication. The primary role of the sac is to act as an amplifier. This helps the male's advertisement call reach much further distances. Because of this, scientists can often use the sac to determine a frog's sex. 
The mechanism of sound production involves several precise steps. First, the frog inflates its lungs with air. To direct the air, the frog shuts both its nose and its mouth. Next, the frog expels the air from its lungs. The air travels through the larynx, which is the part that creates vibrations. This air then moves into the vocal sac. The vibrations of the larynx cause the elastic membrane of the sac to resonate. This resonance provides amplification for the call. 
Muscles within the body wall play a key role in this process. These muscles force the air to move back and forth. The air cycles continuously between the lungs and the vocal sac. This movement allows the frog to maintain its call. The vocal sac is physically connected to the mouth cavity. It features two slits located on either side of the tongue. This structure allows the air to flow freely during the calling process.
Development of the vocal sac varies between different species. However, many follow a similar biological pattern. A unilobular vocal sac, which means a single-lobed sac, begins as two small growths. These growths appear on the floor of the mouth. They grow into two small pouches. These pouches expand until they eventually meet in the center of the mouth. At this point, they form one large cavity. This cavity continues to grow until the sac is fully developed.
The purpose of the sac is largely tied to mating success. The advertisement call is meant to attract females from a large area. The difference in volume between species is quite significant. Frogs without vocal sacs may only be heard within a radius of a few meters. In contrast, some species with vocal sacs can be heard from much further away. This distance is vital for finding mates in large environments.
Some modern frog species do not possess vocal sacs at all. These include many neobatrachians and some mesobatrachians. These specific frogs often live in habitats close to flowing water. The constant sound of moving water can overpower a frog's call. Because the water is so loud, these frogs must use other methods to advertise. This shows how the environment influences the physical traits of a species.
Certain frogs use the vocal sac for a purpose other than sound. This occurs in the family Rhinodermatidae. In these species, males scoop recently hatched tadpoles into their mouths. The tadpoles then move into the vocal sac. The two species in this family handle the young differently. In Darwin's frog, the tadpoles remain in the sac until metamorphosis. Metamorphosis is the process where they change into adults. However, the Chile Darwin's frog will transport the tadpoles to a water source. 
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