Some living things are hard to group. 

Some living things are hard to group. 


Scientists use groups to organize all living things. They use a system called taxonomy to do this. This system is like a big family tree. 


Scientists use a system called taxonomy to organize all living things. This system works like a giant family tree. Every animal, plant, or tiny cell has a specific spot on this tree. Sometimes, a scientist finds something that does not fit anywhere. They might find a new fossil or a strange tiny organism. When they cannot find the right group, they use a term called incertae sedis. This is a Latin phrase that means "of uncertain position." 
There are several reasons why a living thing might be incertae sedis. One reason is a poor description from the start. If the first person to find it did not provide enough details, it is hard to place. Another reason is missing clues in fossils. A fossil might only be a single tooth or a small bone. Without more of the body, the whole animal remains a mystery. Scientists also use this term when they lack DNA. It is hard to get tissue from very rare organisms. They prefer not to guess the wrong spot.
History shows us that many things were once incertae sedis. New World vultures were once in this uncertain group. Scientists did not know which order they belonged to. Later, they were placed in a group called Cathartiformes. 
There are many different examples of these mystery groups in nature. The frosted phoenix moth is so rare that no family is known for it. There is also a single-celled organism called Parakaryon myojinensis. It has a position on the tree of life that is completely unknown. 
Think of the tree of life like a giant library. Every species is a book that needs a specific shelf. If a book has no title or cover, you cannot find its place. You might put it in a special box marked "unknown" for now. 
In the study of biology, scientists use a system called taxonomy to organize every living thing. This system creates a complex hierarchy, much like a massive family tree. Every species, from a tiny bacterium to a giant whale, has a specific place within this tree. However, sometimes a discovery does not fit neatly into any existing branch. When a scientist cannot determine the correct taxonomic position for a group, they use the term *incertae sedis*. This is a Latin phrase that translates to "of uncertain position." 
There are several scientific reasons why a taxon might be labeled *incertae sedis*. One common cause is a poor or inadequate description. If the original researcher did not provide enough physical details, later scientists may struggle to categorize it. Another issue is the presence of extensive homoplasies. This occurs when different species evolve similar traits independently, which can mislead researchers during analysis. Additionally, apparent character state reversals or convergent evolution can make a species look more like one group when it actually belongs to another. These complexities create confusion that prevents a certain classification.
In the field of paleontology, the lack of physical evidence often leads to uncertainty. Many fossils are defined based only on partial information. For example, if a phylogenetic analysis—a study of evolutionary relationships—is built using soft tissue and vertebrae, a fossil consisting only of a single tooth cannot be placed accurately. Without more body parts to study, scientists cannot guess the correct position. They choose to label the taxon *incertae sedis* rather than risk an incorrect assignment. This is also true in modern molecular phylogenies. It is often very difficult to obtain tissue samples from rare organisms for DNA testing. 
Taxonomic uncertainty can also arise from scientific controversy. When researchers produce conflicting results, there may be no consensus on how a taxon relates to other organisms. Until the conflict is resolved through further study, the group remains *incertae sedis*. It is important to distinguish this from other types of uncertainty. For instance, *nomen dubium* refers to a name of unknown application, often because the original specimen is lost. Similarly, *cf.* is used in paleontology to suggest a specimen should be compared to a known species but is not a certain match. *Incertae sedis* specifically addresses the unknown evolutionary position, not just a confusing name.
History provides many examples of organisms that were once *incertae sedis* but were later classified. New World vultures, such as the California condor, were once placed in the class Aves *incertae sedis*. Scientists were unsure of their specific order until the order Cathartiformes was recognized. 
Today, many strange and unique organisms still occupy this uncertain status. The single-celled organism *Parakaryon myojinensis* has a position on the tree of life that is completely unknown. There is also "biological dark matter," which consists of genetic material from unidentified microorganisms that match no known species. In the world of insects, the frosted phoenix moth is so rare and obscure that it cannot be placed in any family within the Lepidoptera. Even ancient groups like the Ediacaran biota have an unclear position within Eukaryota. They might be related to fungi, algae, or perhaps even an entirely extinct kingdom. 
The scale of this uncertainty is particularly large in the study of fungi. In 2022, the "Outline of Fungi and fungus-like taxa" reported approximately 3,185 genera as *incertae sedis*. This includes roughly 2,680 genera within the Ascomycota group and about 420 within the Basidiomycota group. Many of these fungi are well-described physically, yet they still do not match any recognized family or order. This backlog is caused by limited sampling and discordant signals from different genetic markers. As scientists continue to collect fresh samples and perform molecular studies, they will gradually move these mysteries from the "uncertain" box into their rightful places on the tree of life.
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