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Fluorescence

physical science Maturity 9-11 Next article →

Fluorescence is a specific type of photoluminescence, which is the emission of light by a substance that has absorbed electromagnetic radiation. While many substances can emit light, fluorescence is unique because it occurs almost immediately upon excitation. When these materials are exposed to radiation, such as ultraviolet (UV) light, they glow with visible colors. The specific color emitted depends entirely on the chemical composition of the substance.

Fluorescent minerals hg
Fluorescent minerals hg

This phenomenon is vital in many fields, from medical diagnostics to modern lighting technology. It allows us to detect tiny biological structures or create efficient white light for our homes.

The mechanism of fluorescence begins at the molecular level. It starts when a molecule absorbs an incoming photon from radiation. This absorption pushes the molecule to a higher, more energetic state called an excited state.

Jablonski Diagram of Fluorescence Only-en
Jablonski Diagram of Fluorescence Only-en

Most of these excited states are singlet states, meaning the electronic spin of the electrons remains the same. To return to its stable, lower energy state, the molecule must release this extra energy. It does this by emitting a new photon of light. This emitted light often has a longer wavelength and lower energy than the original radiation. For example, invisible UV light can be absorbed and then emitted as visible colored light.

Ruby ball fluorescence @ 520nm laser illumination
Ruby ball fluorescence @ 520nm laser illumination

Fluorescence is distinct from other types of light emission, most notably phosphorescence. In fluorescence, the molecule returns to its ground state quickly, often within nanoseconds. This is a result of the electronic spin multiplicity remaining the same. In contrast, phosphorescence involves a change in the electron's spin, moving it into a triplet state. This change makes the return to the ground state much slower. While fluorescence stops nearly instantly when the light source is removed, phosphorescence can create a visible afterglow. This afterglow can last from a few microseconds to many hours.

Stokes shift- Rh6G
Stokes shift- Rh6G

Humanity has observed these glowing effects for centuries, even before the science was fully understood. The Aztecs were aware of certain glowing properties long ago. In the mid-1500s, observers like Bernardino de Sahagún and Nicolás Monardes described a glowing infusion called lignum nephriticum. This substance was derived from the wood of trees like Pterocarpus indicus and Eysenhardtia polystachya. The glow came from a chemical called matlaline. In 1852, George Gabriel Stokes officially named the phenomenon "fluorescence." He chose this name because it was analogous to the mineral fluorite, or fluorspar.

Lignum nephriticum - cup of Philippine lignum nephriticum, Pterocarpus indicus, and flask containing its fluorescent solution Hi
Lignum nephriticum - cup of Philippine lignum nephriticum, Pterocarpus indicus, and flask containing its fluorescent solution Hi

Scientists use various measurements to understand how efficient a fluorescent material is. One key measurement is the fluorescence quantum yield. This is the ratio of the number of photons emitted to the number of photons absorbed. A quantum yield of 1.0 means every single absorbed photon resulted in an emitted photon. Other factors can lower this efficiency through non-radiative processes. These include internal conversion, where energy is lost as heat, or collisional quenching. Quenching happens when another molecule, like molecular oxygen, collides with the excited molecule and steals its energy.

US $20 under blacklight
US $20 under blacklight

Today, fluorescence has massive practical significance in technology and science. It is used in medicine for fluorescent labeling and in chemical sensors through fluorescence spectroscopy. It is also essential for vacuum fluorescent displays and cathode-ray tubes. One of the most common uses is in gas-discharge fluorescent lamps and LED lamps. In these devices, fluorescent coatings convert UV or blue light into longer wavelengths. This process creates the white light used in everyday lighting.

Fluoreszenz-6l6G hg
Fluoreszenz-6l6G hg

In high-tech applications like lasers, scientists rely on the extremely fast decay times of fluorescence, which can occur in the nanosecond range.

Fluorescence also appears frequently in the natural world through a process called biofluorescence. This occurs when a fluorophore is part of or derived from a living organism. Many marine organisms exhibit this, including various species of coral and fish.

Some jellyfish, such as Aequorea victoria, are famous for their biofluorescent properties. On land, fluorescence can be seen in animals like certain scorpions or polka-dot tree frogs.

Hypsiboas punctatus fluorescente
Hypsiboas punctatus fluorescente

These biological traits show how deeply the chemical properties of fluorescence are integrated into the various kingdoms of life.

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🖼️ Images & Media (23)
File:Fluorescent minerals hg.jpg
Fluorescent minerals hg.jpg
File:Adaptive-Evolution-of-Eel-Fluorescent-Proteins-from-Fatty-Acid-Binding-Proteins-Produces-Bright-pone.0140972.g001.jpg
Adaptive-Evolution-of-Eel-Fluorescent-Prot...
File:Black light theatre Prague HILT 13.jpg
Black light theatre Prague HILT 13.jpg
File:Lignum nephriticum - cup of Philippine lignum nephriticum, Pterocarpus indicus, and flask containing its fluorescent solution Hi.jpg
Lignum nephriticum - cup of Philippine...
File:Matlaline structure.svg
Matlaline structure.svg
File:Ruby ball fluorescence @ 520nm laser illumination.jpg
Ruby ball fluorescence @ 520nm laser...
File:Fluoreszenz-6l6G hg.jpg
Fluoreszenz-6l6G hg.jpg
File:Jablonski Diagram of Fluorescence Only-en.svg
Jablonski Diagram of Fluorescence Only-en.svg
File:US $20 under blacklight.jpg
US $20 under blacklight.jpg
File:Stokes shift- Rh6G.png
Stokes shift- Rh6G.png
File:Fluorescent Coral Movie.gif
Fluorescent Coral Movie.gif
File:Observed occurrences of green and red biofluorescence in Actinopterygii - journal.pone.0083259.g002.png
Observed occurrences of green and red...

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