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Biofilm

life science Maturity 9-11 Next article →

A biofilm is a complex, syntrophic community of microorganisms. In these groups, cells stick to one another and often attach to a surface.

biofilm
biofilm

These cells become embedded in a slimy material called an extracellular polymeric substance, or EPS. This matrix is a combination of polysaccharides, proteins, lipids, and DNA. Because biofilms have a three-dimensional structure, scientists often call them "cities for microbes." This comparison reflects how they function as a coordinated, living system.

Mature biofilm structure
Mature biofilm structure

Biofilms can exist on both biotic, or living, and abiotic, or non-living, surfaces.

The formation of a biofilm follows a specific sequence of events. It begins when free-floating, or planktonic, bacteria attach to a surface.

Escherichia coli forming biofilms via F-pilus
Escherichia coli forming biofilms via F-pilus

Initially, these first colonists use weak van der Waals forces or hydrophobic effects to stick. To stay anchored, they may use specialized structures like pili. Some Archaea even use hami, which are long tubes with three hooks.

biofilm
biofilm

Once attached, the bacteria undergo a phenotypic shift. This means they change their behavior by regulating large groups of genes. The biofilm then grows through cell division and the recruitment of new cells.

Biofilm development is categorized into five major stages. The first is initial attachment, followed by irreversible attachment.

biofilm
biofilm

After these, the community enters Maturation I and then Maturation II. In these stages, the biofilm grows into a complex structure. The final stage is dispersal, which is essential for the life cycle.

Honors Option-MMG
Honors Option-MMG

During dispersal, cells leave the colony to colonize new surfaces. This process is complex and involves specific enzymes or chemical messengers. Some bacteria use nitric oxide or fatty acids to trigger this departure.

The extracellular matrix is much more than just simple slime. It is a hydrogel, which is a polymer that holds many times its dry weight in water.

biofilm
biofilm

This matrix facilitates communication through biochemical signals and allows for gene exchange. It can also act as an external digestion system by trapping enzymes near the cells. Some biofilms even feature water channels to distribute nutrients and signals. The matrix may also trap environmental materials like minerals, soil, or blood components. In some cases, the matrix is strong enough to become fossilized as stromatolites.

Biofilms have a very long history on our planet. Scientists believe they arose on primitive Earth as a defense mechanism. At that time, environmental conditions were often too harsh for single cells to survive.

We find evidence of biofilms in the fossil record dating back 3.25 billion years. These ancient fossils contain both Bacteria and Archaea. By providing homeostasis, biofilms encouraged the development of complex interactions between cells. This ancient survival strategy has remained successful for billions of years.

Living in a biofilm provides significant advantages over a planktonic lifestyle. One major benefit is increased resistance to environmental threats. The dense matrix acts as a physical barrier against toxins, antibiotics, and predators.

Staphylococcus aureus biofilm 01
Staphylococcus aureus biofilm 01

In some instances, this protection can make a biofilm up to 5,000 times more resistant to antibiotics. Cells also use quorum sensing to communicate.

biofilm
biofilm

This is a process where bacteria use chemical products, like N-acyl homoserine lactone, to sense their population density. This communication allows them to coordinate activities like virulence or matrix production.

Biofilms are found in many diverse environments. In nature, they can be seen as colorful mats in Yellowstone National Park hot springs.

Bacteria mats near Grand Prismatic Spring in Yellowstone
Bacteria mats near Grand Prismatic Spring in Yellowstone

They can also form in caves, such as the golden hydrophobic bacteria in Golden Dome Cave.

A114, Lava Beds National Monument, California, USA, Golden Dome Lava Tube Cave, 2004
A114, Lava Beds National Monument, California, USA, Golden Dome Lava Tube Cave, 2004

On animals, biofilms appear as dental plaque on teeth. This can lead to gum disease or tooth decay. They can even be found in extreme environments like the Dead Sea.

Screen Shot 2017-12-13 at 1.40.19 PM
Screen Shot 2017-12-13 at 1.40.19 PM

These communities show how microbes can adapt to almost any setting.

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🖼️ Images & Media (11)
File:Staphylococcus aureus biofilm 01.jpg
Staphylococcus aureus biofilm 01.jpg
File:Algae in silicified sediment with evaporite crystals. Urgonian carbonate platform of Provence. 1.jpg
Algae in silicified sediment with...
File:A114, Lava Beds National Monument, California, USA, Golden Dome Lava Tube Cave, 2004.jpg
A114, Lava Beds National Monument,...
File:Mature biofilm structure.png
Mature biofilm structure.png
File:biofilm.jpg
biofilm.jpg
File:Honors Option-MMG.svg
Honors Option-MMG.svg
File:Mixed-culture biofilm.jpg
Mixed-culture biofilm.jpg
File:Bacteria mats near Grand Prismatic Spring in Yellowstone.JPG
Bacteria mats near Grand Prismatic Spring...
File:Thermophilic bacteria.jpg
Thermophilic bacteria.jpg
File:Screen Shot 2017-12-13 at 1.40.19 PM.png
Screen Shot 2017-12-13 at 1.40.19 PM.png
Escherichia coli forming biofilms via F-pilus.tif
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