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Anabaena

life science Maturity 11-13 Next article →

Anabaena is a genus of filamentous cyanobacteria that exist as plankton. These tiny organisms live in long, chain-like structures called filaments. They are highly significant because of their ability to perform nitrogen fixation. This process allows them to convert atmospheric nitrogen into forms that living things can use. They also form important symbiotic relationships with certain plants. One famous example is the partnership with the mosquito fern, known as Azolla.

The most remarkable feature of Anabaena is its specialized cells called heterocysts. These cells are designed specifically for nitrogen fixation. To make this process work, the heterocyst must create a micro-oxic environment. This means it must keep oxygen levels very low. The cell achieves this by increasing respiration and inactivating photosystem II, which is the part that produces oxygen. It also forms a thickened envelope outside the cell wall to act as a barrier.

Inside these protected heterocysts, an enzyme called nitrogenase performs the chemical work. Nitrogenase transforms dinitrogen from the air into ammonia. This process requires a lot of energy in the form of ATP and reductant. This energy comes from carbohydrate metabolism. In the light, the process is supplemented by the activity of photosystem I. The heterocysts rely on the regular vegetative cells for fuel. The vegetative cells produce carbohydrates, likely in the form of glucose, and move them into the heterocysts. In return, the heterocysts send fixed nitrogen back to the vegetative cells, often as amino acids.

Anabaena also engages in complex symbiotic relationships. The fern Azolla hosts the species Anabaena azollae. This partnership allows the fern to access essential nitrogen from the atmosphere. Because of this, Azolla is often called a "super-plant." It can colonize freshwater areas and grow at an incredible speed. The plant can double its biomass in as little as 1.9 days. However, this growth is usually limited by the availability of phosphorus.

In these relationships, the microorganisms are passed directly from one generation of the plant to the next. This has made Anabaena azollae completely dependent on its host. Some of its genes have even been lost or transferred to the nucleus of the Azolla cells. Beyond helping plants, Anabaena is used by scientists to study vision. They study a light-sensitive membrane protein called sensory rhodopsin. By observing how light changes the shape of these molecules, researchers can learn how vision works in vertebrates.

Scientific research has also focused on the genetic makeup of these organisms. Between 1999 and 2005, the United States Department of Energy conducted a DNA sequencing project. They mapped the complete genome of the model organism Anabaena variabilis ATCC 29413. This genome is 7.2 million base pairs long. A paper detailing this work was published in 2014. Researchers also study how Anabaena repairs its DNA. They have looked at how a protein called RecN helps repair double strand breaks. This repair is active in vegetative cells but absent in mature heterocysts.

While Anabaena is useful for rice paddy fields as a natural fertilizer, it can also be dangerous. Anabaena is one of four genera of cyanobacteria that produce neurotoxins. These toxins can be harmful to local wildlife, farm animals, and pets. Scientists assume the production of these neurotoxins is an input into symbiotic relationships. The toxins likely protect the host plant from grazing pressure by making it undesirable to eat. This shows how even microscopic organisms can deeply affect entire ecosystems.

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